The Influence of Vitamin B12 on The Progression of Fracture Healing in Rats.
The Influence of Vitamin B12 on The Progression of Fracture Healing in Rats.
1*Mana, H. P., 2,4 Mutah, A. A., 3Mohzo, D. L., 2Hermon, W.
1Veteriary Teaching Hospital, University of Maiduguri, PMB 1069, University of Maiduguri, Nigeria;
2Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, PMB 1069, University of Maiduguri, Nigeria;
3Department of Veterinary Pathology, Faculty of Veterinary Medicine, PMB 1069, University of Maiduguri, Nigeria.
4Bioengineering Department, Sinclair Lab, 7 Asa Drive, Suite 205 Bethlehem, PA 18015 USA.
Publication Information
Journal Title: International Journal of Research and Scientific Innovation (IJRSI)
Author(s): H. P.,Mana; A. A.,Mutah; D. L.,Mohzo;W.,Hermon
Published On: 08/21/2025
Volume: 11
Issue: 6
First Page: 382
Last Page: 391
ISSN: 2321-2705
- Cite this Article H. P.,Mana; A. A.,Mutah; D. L.,Mohzo;W.,Hermon ; The influence of Vitamin B12 on the Progression of Fracture Healing in Rats.
, Volume 11 Issue 6, International Journal of Research and Scientific Innovation (IJRSI),382-391, Published on 08/21/2025, Available at https://rsisinternational.org/journals/ijrsi/articles/the-influence-of-vitamin-b12-on-the-progression-of-fracture-healing-in-rats/
Abstract
Bone fracture is a complete or partial breakage of the bone structure. Bone fracture occurs when the bone structure is either completely or partially broken. The process of healing involves the regeneration of new bone tissue, which seamlessly integrates with the uninjured bone. Fracture repair entails rejoining and realigning the broken bone ends to facilitate healing. Previous reparative studies to enhance repair were limited in incorporating a therapeutically active factor to facilitate repair. Cyanocobalamin, a synthetic form of vitamin B12, is readily converted in the body into its active forms, methylcobalamin and cobamamide. In this study, 30 rats were obtained from a reputable breeder (University of Maiduguri staff quarters, line F house No. 1), acclimatized for 21 days, and divided into two groups: Group A, receiving vitamin B12 (15 rats), and Group B, serving as the control (15 rats) recived placebo (normal saline). Group A received a dosage of vitamin B12 at 500 micrograms/kg, while Group B remained untreated. Samples from fractured tibiae were collected at two weeks intervals on days 14, 28, and 42, respectively. These samples were decalcified using formic acid, stained with Hematoxylin and Eosin (H&E), and examined under a microscope at magnifications of X10, X40, and X100. We used qualitative data analysis method to analyse our results. However, based on qualitative analysis, there were noticeable gross and histological disparities on days 28 and 42. These findings suggest that vitamin B12 supplementation enhances collagen formation on days 28 and 42, potentially improving fracture repair using the qualitative data analysis method.
Keywords: Fracture, Rats, Vitamin B12, Histology, Collagen
